Abstract Objectives: Depression is common after both lacunar stroke and non-lacunar stroke and might be associated with lesion locations as proven by some studies. This study aimed to identify whether lesion location was critical for depression after both lacunar and non-lacunar strokes. Methods: A cohort of ischemic stroke patients was assigned to either a lacunar stroke group or a non-lacunar stroke group after a brain MRI scan. Neurological deficits and treatment response was evaluated during hospitalization. The occurrence of depression was evaluated 3 months later. Logistic regressions were used to identify the independent risk factors for depression after lacunar and non-lacunar stroke respectively. Results: 83 of 246 patients with lacunar stroke and 71 of 185 patients with non-lacunar stroke developed depression. Infarctions in the frontal cortex, severe neurological deficits, and a high degree of handicap were identified as the independent risk factors for depression after non-lacunar stroke, while lesion location was not associated with depression after lacunar stroke. Conclusion: The main determinants for depression after lacunar and non-lacunar stroke were different. Lesion location was critical only for depression after non-lacunar stroke.

Abstract The construction of the cross passage changes the main tunnels segment from a complete cylindrical structure to a notched unstable stressed structure. Thus, the overall force (bending and shearing) performance of the main tunnels is considerably decreased. The excavation of the cross passage also destroys the stability of the original soil layer and main tunnels and causes difficulty in avoiding the settlement of the surface and main tunnels. Numerical simulation analysis of the mechanical characteristics and influencing factors analysis were carried out using FLAC3D. Results show that (1) the construction of the cross passage largely disturbs the main tunnels. The settlement of the intersection part of the main tunnels and cross passage is mainly concentrated from −7 m to 7 m. (2) During the construction of the cross passage, the original main tunnels are subjected to stress redistribution, which causes stress concentration in the intersecting segments, especially in the opening side segment has an unfavorable force form in which the upper and lower sections are integrally pulled. (3) The construction of the cross passage causes a large tensile stress area in the structure as well. As a result, the overall tension of the upper and lower sections of the structure and the compression of the hance are unfavorable. (4) The influence of the excavation of the cross passage on the surface and main tunnels is related to the buried depth, intersection angle, excavation method, and grouting range. Therefore, the influence of different parameters can be analyzed by changing the model parameters.

A simple and effective salting-out method was developed for the purification of the metallo-β-lactamase CcrA from Bacteroides fragilis based on the plasmid pMSZ02, in which the crude protein secreted into growth medium was precipitated by 80% sulfate saturation of the medium and purified with Q-Sepharose to offer pure CcrA with yield of 20.1 mg per litter medium. The dependence of the amount of protein precipitation on sulfate saturation was investigated, which showed that more than 80% sulfate saturation resulted the maximum protein precipitated. The purified CcrA was evaluated by steady-state kinetics using penicillin G and cephalothin V as substrates, which showed the Km values of 68±2 and 17±2 µM and Kcat values of 63±1 and 102±3 S-1, respectively. The comparison with the data of the protein from literature method showed that the salting-out method was viable, and it could be useful for the purification of other proteins secreted into growth medium.